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fluorescence polarization immunoassay for alternaria mycotoxin tenuazonic acid detection and molecular modeling studies of antibody recognition
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نویسنده
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wang feng ,cai jun ,eremin sergei a. ,xiao zhi-li ,shen yu-dong ,tian yuan-xin ,xu zhen-lin ,yang jin-yi ,lei hong-tao ,sun yuan-ming ,wang hong
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منبع
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food analytical methods - 2018 - دوره : 11 - شماره : 9 - صفحه:2455 -2462
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چکیده
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A simple and rapid method of fluorescence polarization immunoassay (fpia) for detection of alternaria mycotoxin tenuazonic acid (tea) was described. in this study, the tracers teah-fitc and teah-dtaf were synthesized from hapten 5-(sec-butyl)-3-(1-hydrazonoethyl)-4-hydroxy-1h-pyrrol-2(5h)-one (teah) and different fluoresceins isothiocyanate isomer i (fitc) or 5-([4,6-dichlorotriazin-2-yl]amino)fluorescein hydrochloride (dtaf), respectively. the established fpia showed an ic50 value of 3.0 μg/ml with a detection limit of 0.13 μg/ml for teah based on the tracer of teah-fitc, and with the same properties of 7.3 and 0.93 μg/ml based on teah-dtaf. the former gave better assay sensitivity than the latter, that the 2,4-dichloro-s-triazine group of teah-dtaf affect the specific recognition of tracer and antibody. models of the lowest energy conformation and the molecular electrostatic potential isosurface studies revealed that the –c=n–n– and –chch3(ch2ch3) groups were the characteristic chemical groups in anti-teah antibody-analyte recognition via molecular modeling, which could create a theoretical guide for hapten design of small molecules and provide the traditional theory with empirical proof.
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کلیدواژه
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tenuazonic acid ,fluorescence polarization immunoassay ,molecular modeling ,antibody recognition
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آدرس
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south china agricultural university, college of food science, national-local joint engineering research center for processing and safety control of livestock and poultry products, guangdong provincial key laboratory of food quality and safety, people’s republic of china, south china agricultural university, college of food science, national-local joint engineering research center for processing and safety control of livestock and poultry products, guangdong provincial key laboratory of food quality and safety, people’s republic of china, m.v. lomonosov moscow state university, faculty of chemistry, russia, south china agricultural university, college of food science, national-local joint engineering research center for processing and safety control of livestock and poultry products, guangdong provincial key laboratory of food quality and safety, people’s republic of china, south china agricultural university, college of food science, national-local joint engineering research center for processing and safety control of livestock and poultry products, guangdong provincial key laboratory of food quality and safety, people’s republic of china, southern medical university, school of pharmaceutical sciences, people’s republic of china, south china agricultural university, college of food science, national-local joint engineering research center for processing and safety control of livestock and poultry products, guangdong provincial key laboratory of food quality and safety, people’s republic of china, south china agricultural university, college of food science, national-local joint engineering research center for processing and safety control of livestock and poultry products, guangdong provincial key laboratory of food quality and safety, people’s republic of china, south china agricultural university, college of food science, national-local joint engineering research center for processing and safety control of livestock and poultry products, guangdong provincial key laboratory of food quality and safety, people’s republic of china, south china agricultural university, college of food science, national-local joint engineering research center for processing and safety control of livestock and poultry products, guangdong provincial key laboratory of food quality and safety, people’s republic of china, south china agricultural university, college of food science, national-local joint engineering research center for processing and safety control of livestock and poultry products, guangdong provincial key laboratory of food quality and safety, people’s republic of china
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Authors
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