تولید فیلم مرکب ژلاتین - پلولان- نانو الیاف سلولز حاوی فاژ سالمونلا و بررسی اثر ضدسالمونلایی آن علیه سالمونلا تایفی موریوم

امروزه به‌ دلیل وجود نگرانی‌ های زیست‌ محیطی و افزایش تقاضای مصرف‌کنندگان برای محصولات غذایی با کیفیت و ماندگاری بیشتر، استفاده از فیلم‌ها و پوشش‌های زیست تخریب‌ پذیر مورد توجه بسیاری واقع شده‌اند. در این مطالعه، اثر افزودن درصدهای مختلف نانوالیاف سلولز به فیلم مرکب ژلاتین-پلولان و اثر ضدباکتریایی فیلم‌های حاوی باکتریوفاژ توسط روش انتشار دیسک بررسی شد. به‌علاوه، اثر ضدباکتریایی فیلم مرکب ژلاتین-پلولان-نانو فیبر سلولز روی گوشت مرغ در طول دوره نگه‌داری در دو دمای 4 و 12 درجه سانتی‌گراد علیه باکتری سالمونلا تایفی موریوم مورد مطالعه قرار گرفت. نتایج نشان داد که با افزایش درصد نانوفیبرهای سلولز در فیلم ژلاتین-پلولان ضخامت، حلالیت، تورم، مقاومت کششی، و درصد کشش پذیری فیلم‌ها به‌ترتیب افزایش، کاهش، افزایش، افزایش، و کاهش یافتند. فیلم‌های حاوی باکتریوفاژ روی محیط آگار ناحیه بازدارندگی خوبی داشتند. استفاده از فیلم ضدباکتریایی روی سطح گوشت مرغ در دمای  12 بعد از یک روز منجر به کاهش یک سیکل لگاریتمی شد در حالی‌که در دمای  4 در روز هفتم یک سیکل لگاریتمی کاهش را در جمعیت باکتری سالمونلا منجر شد.

production of gelatin-pullulan- nanofibers cellulose film containing salmonella phages and effect its anti-salmonella against salmonella typhimurium

the main sources of salmonella for humans are pork, beef, chicken, eggs, fruits, vegetables, and their derivatives such as mayonnaise, and peanut butter. different species of salmonella can adapt, grow or survive at different environmental conditions. salmonella enterica is a majorcause of food borne illness in humans, and salmonella typhimurium and salmonella enteritidis serovars are the most prevalent. one strategy is to use active packaging to reduce the microbial load or prevent the growth of microorganisms on food. recently, antimicrobial active packaging has received much attention due to maintaining food quality, safety, and increasing shelf life. among the antimicrobials used in the food industry, bacteriophages have a very good efficiency to control pathogenic bacteria. pullulan has a good ability to form a film, its film has good characteristics such as transparency, odorlessness, tastelessness, solubility in water, and low permeability to oxygen and fat, however, the major obstacle is related to its price. the combination of polysaccharides with proteins has been done in order to improve the performance and reduce the costs of films. gelatin is a suitable option to combine with pullulan in terms of good mechanical properties, reduced permeability, and its good price. different ratios of gelatin and pullulan were studied and suitable film selected, but it needed to modify, so nanofibers cellulose was added in order to improve the mechanical properties and water resistance. adding cellulose nano fiber can be a good and appropriate option. the aim of this research was to evaluate theantibacterial effectiveness of gelatin-pullulan- nanofibers cellulose composite film containing bacteriophage against salmonella typhimurium at two different temperatures. materials and methodsgelatin and pullulan powders were weighted separately and mixed together (20gelatin-80pullulan). nanofiber cellulose was extracted from rice bran and was used at three different levels (1%, 3%, and 5%). commercial bacteriophage solution was added to each of the films separately and the films were prepared by molding method. thickness, moisture content, solubility, swelling, tensile strength, and elongation of gelatin-pullulan film containing nanofibers were studied. zone inhibitory of films containing different percentage of cellulose nanofibers on the agar media against salmonella typhimurium (104 cfu.ml-1) was evaluated. the, antibacterial effect of selected film on the poultry meat inoculated with s. typhimurium (104 cfu.g-1) and several phages on the surface meat at 4  and 12  was also investigated.