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design & construction of a tapered optical fiber sensor for the detection of streptavidin protein
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نویسنده
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mazlum dizaji b. ,vahedi m. ,maleki a. ,saghafifar h.
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منبع
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پژوهش فيزيك ايران - 1401 - دوره : 22 - شماره : 3 - صفحه:193 -198
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چکیده
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This paper presents the fabrication process and operation of a bio-sensor based on tapered optical fiber by using optical spectrum shift in the presence of pbs buffer and dissolved streptavidin. the taper-fiber sensors are 18 and 24 μm in diameter and are fabricated by use of an oxy-butane torch. the optical spectrum is obtained using a broadband light source and an optical spectrum analyzer (osa) in a 40 nm wavelength range. the existence of streptavidin on the tapered fiber surface is confirmed. the experiments on pbs buffer have shown that, the output spectrum demonstrates a red-shift by adding the streptavidin. the maximum sensitivity obtained is 29000 nm/riu.
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کلیدواژه
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tapered fiber ,biosensor ,streptavidin ,biological buffer
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آدرس
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iran university of science and technology, school of physics, iran, iran university of science and technology, school of physics, iran, iran university of science and technology, catalysts and organic synthesis research laboratory, department of chemistry, iran, malek ashtar university of technology, optics & laser science and technology research center, iran
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پست الکترونیکی
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saghafifar@mut-es.ac.ir
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design & construction of a tapered optical fiber sensor for the detection of streptavidin protein
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Authors
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Abstract
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this paper presents the fabrication process and operation of a bio-sensor based on tapered optical fiber by using optical spectrum shift in the presence of pbs buffer and dissolved streptavidin. the taper-fiber sensors are 18 and 24 μm in diameter and are fabricated by use of an oxy-butane torch. the optical spectrum is obtained using a broadband light source and an optical spectrum analyzer (osa) in a 40 nm wavelength range. the existence of streptavidin on the tapered fiber surface is confirmed. the experiments on pbs buffer have shown that, the output spectrum demonstrates a red-shift by adding the streptavidin. the maximum sensitivity obtained is 29000 nm/riu.
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Keywords
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tapered fiber ,biosensor ,streptavidin ,biological buffer
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