Preliminary results of treating cancerous cells of lung (QU-DB) by hyperthermia using diode laser and gold coated Fe3O4/SiO2 nano-shells: An in-vitro assay

Introduction: in this study,we describe the results of controlled synthesis and application of gold coated fe3o4/sio2 nanoshells combined with the optical property of gold for enhancement of selective photothermal interaction with cancerous cells based on the surface plasmon resonance (spr) phenomena. materials and methods: magnetite nano-particles (mnps) were prepared by means of co-precipitation. mnps were modified with a thin layer of silica using the stober method. the amino-modified fe3o4/sio2 nano-shells were covered with gold colloids as a self-assembeled process. in-vitro assays were performed to determine the effect of apoptosis of the cells based on the cells morphological changes. results: the biologically inert nano-shells (85 nm) with a magnetite/silica core and a gold shell were optically activated. a successful laser-hyperthermia based on the thermal effect of surface plasmon resonance was performed using different gold concentrations. the thermal profile effects of laser power are presented as ideal cases of nanoshell-assisted photo-thermal therapy. the thermally-induced cell death has been shown to be dependent on nps concentration and laser power density. the power densities of 157 and 184 w/cm2 caused complete cell death at the focal point of the laser beam. cell damage was reduced by decreasing the power density of laser. also,a larger area of damage on cell culture plates was observed at longer intervals of laser irradiation. conclusion: an optimized laser-(spr) hyperthermia was obtained using a concentration of gold coated fe3o4/sio2 nanoshells concentration=0.1 mg/ml at intensity=157 w/cm2 at 60s.