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modified methods to simplification histochemical, immunohistochemical, and hematoxylin-eosin staining
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نویسنده
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kalantari-hesari ali ,morovvati hassan ,babaei mohammad ,nourian alireza ,esfandiari kave ,elmi taher ,soltani salman
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منبع
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دامپزشكي ايران - 1401 - دوره : 18 - شماره : 3 - صفحه:63 -73
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چکیده
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Most histotechnical methods and preparation of tissue sections were invented and used in the 18th and 19th centuries. due to the colorless nature of most cells and tissues in the body, tissue staining methods were used from the beginning to differentiate cells and tissues. materials methods: according to sources and reports in this field, the time required for each hematoxylin-eosin staining period is approximately 80 minutes. in the present study, a method of hematoxylin-eosin staining is reported that reduces the time required for staining by maintaining the quality of sections and staining from 80 minutes to less than 40 minutes. also, considering the existing problems regarding the cost of histochemistry and immunohistochemistry kits, the low amount of kits and the problems that exist in the drip staining method of this type of staining, the present report tries to present three creative methods in this regard. these methods increase the quality of the prepared slides, in addition to reducing consumables. optimization of tissue staining steps can reduce expenses spent and increase the quality of tissue sections.
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کلیدواژه
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tissue sections ,histotechnique ,histochemistry ,immunohistochemistry
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آدرس
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bu-ali sina university, faculty of veterinary science, department of pathobiology, iran, university of tehran, faculty of veterinary medicine, department of basic science, iran, bu-ali sina university, faculty of veterinary science, department of clinical sciences, iran, bu-ali sina university, faculty of veterinary science, department of pathobiology, iran, science and research branch, islamic azad university, faculty of veterinary medicine, department of basic science, iran, babol branch, islamic azad university, department of laboratory science, iran, university of ilam, central laboratory, iran
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پست الکترونیکی
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soltani1981@yahoo.com
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modified methods to simplification histochemical, immunohistochemical, and hematoxylin-eosin staining
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Authors
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Abstract
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most histotechnical methods and preparation of tissue sections were invented and used in the 18th and 19th centuries. due to the colorless nature of most cells and tissues in the body, tissue staining methods were used from the beginning to differentiate cells and tissues. materials methods: according to sources and reports in this field, the time required for each hematoxylin-eosin staining period is approximately 80 minutes. in the present study, a method of hematoxylin-eosin staining is reported that reduces the time required for staining by maintaining the quality of sections and staining from 80 minutes to less than 40 minutes. also, considering the existing problems regarding the cost of histochemistry and immunohistochemistry kits, the low amount of kits and the problems that exist in the drip staining method of this type of staining, the present report tries to present three creative methods in this regard. these methods increase the quality of the prepared slides, in addition to reducing consumables. optimization of tissue staining steps can reduce expenses spent and increase the quality of tissue sections.
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Keywords
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tissue sections ,histotechnique ,histochemistry ,immunohistochemistry
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