a rapid method for detection of crimean congo hemorrhagic fever virus with loop-mediated isothermal amplification assay

Background :crimean congo hemorrhagic fever virus (cchfv) is an extremely virulent pathogen, and ticks are a reservoir of the virus. one of the cchfv endemic countries is iran, where human cases of the infection have been reported in nearly all parts of the country. objectives :in the present study, a reverse transcription loop-mediated isothermal amplification (rt-lamp) assay was designed for rapid detection of the small (s) rna segment of cchfv. methods :a set of rt-lamp primers from the highly conserved region of the s segment was designed for identification of cchfv strains. the rt-lamp, under isothermal conditions at 63°c, was performed without any superior equipment. results :the sensitivity results indicated that rt-lamp detected 62 fg of cchfv rna. a 100% agreement between the results of the rt-lamp and rt-polymerase chain reaction (pcr) was observed. conclusions :this method is more economical and practical than other molecular detection methods such as real-time pcr and nested pcr in developing countries. this method is suitable for rapid detection of this disease in rural hospitals and remote areas.