evaluation of mutation in spike gene of covid-19 in iranian patients

Sars-cov- 2, originated at wuhan (china), has spread rapidly worldwide. mutant variants have been identified in various countries. in this study, samples of 18 covid-19 cases in the third wave and 25 in the fourth wave of the epidemic in iran were collected and sequenced using the sanger method. the results showed different mutations in the third and fourth waves of the epidemic. in the fourth wave, dominant mutations were e484k, d614g, and s519f, which were previously observed in another country. this study showed that the sars-cov-2 genome is constantly going under changes, and evaluating and monitoring mutations in the virus genome is necessary to control and eradicate the disease.