A Novel Electrochemical Sensor Based on MnO2/Sepiolite Nanocomposite for the Detection of Hydrogen Peroxide in Human Serum Samples

Background: the reliable and easy-to-operate detection of hydrogen peroxide (h2o2) has attracted extensive attention in the fieldsof biomedicine, food security, and environmental analysis.objectives: in this work, a novel electrochemical method was proposed for h2o2 monitoring using a carbon paste electrode (cpe)modified with mno2/sepiolite nanocomposite.methods: mno2/sepiolite material was characterized by transmission electron microscopy (tem), energy-dispersive x-ray spectroscopy(eds), and x-ray diffraction (xrd) technique. the modified cpe was employed for the amperometric monitoring of h2o2in human serum samples.results: electrochemical data showed that the mno2/sepiolite-cpe displays a high peak current towards h2o2 oxidation. a linearrange from 5 to 700 µm and a low detection limit of 0.8µm for h2o2 were obtained with the proposed sensor. besides, the electrodedepicted excellent reproducibility and anti-interferant ability, promising the applicability of this electrochemical method inpractical analyses.conclusions: this work introduced a new and effective enzyme-lessh2o2 sensor based on themno2/sepiolite nanocomposite modifiedcpe. the suggested sensor showed good sensitivity for the rapid detection of h2o2 in a wide linear range with a low detectionlimit and satisfactory reproducibility, which made it practical for the analysis of hydrogen h2o2 in real samples.