Histological Evaluation Ofwound Healing Process After Photodynamic Therapy of Rat Oral Mucosal Ulcer

Statement of the problem: when the body defense is compromised, wounds can act as a route for entrance and colonization of microorganisms in the body. photodynamic therapy with methylene blue is known as a promising antimicrobial modality. purpose: the present study aimed to investigate the effects of this procedure on wound healing processes. materials and method: in this experimental study, 48 male wistar rats were recruited. experimental wounds were surgically made on their buccal mucosa. based on the treatment modality, they were divided into 3 groups (n=16) of control (cg), laser (lg), photosensitizer+ laser (plg) by methylene blue (mb). the treatment procedure in the two latter groups was done in days 1-4 and 6-9. after sacrificing on 2, 4, 7 and 14-day follow-ups, the microscopic grade of healing of the wounds was assigned on each interval according to histological grading criteria. results: a qualitative result was obtained that showed a healing progression in plg at day 2 of follow-up. at day 4 of follow-up, no difference was seen in healing stage among the groups. however on day 7 of follow-up, samples of the lg showed a lower degree of healing compared with the other two groups. likewise, on day 14 of followup, both plg and lg showed lower degree of healing than cg. conclusion: this study qualitatively showed that mb- mediated photodynamic therapy would have an inhibitory effect on healing process after 14 days of the wound creation.