Evaluation of Shear Bond Strength of Total- and Self-Etching Adhesive Systems After Application of Chlorhexidine To Dentin Contaminated With A Hemostatic Agent

Statement of the problem: hemostatic agents may influence the bond strength of different bonding agents. also, chlorhexidine has shown positive effects on bond strength values and their combination effect has not been reported yet.purpose: the aim of this study was to evaluate the effect of contamination with a hemostatic agent on shear bond strength (sbs) of total- and self-etching adhesive systems and the effect of chlorhexidine application after removal of the hemostatic agent.materials and method: in this experimental study, the occlusal enamel of each sixty caries-free mandibular molars was removed and their midcoronal dentin was exposed. the specimens were then mounted in auto-polymerizing resin 1mm apical to cej. then, the specimens were divided into 6 groups (n=10) based on contamination with a hemostatic agent (h), application of chlorhexidine (chx) and the adhesive system used; and then were classified as group 1: adper single bond (asb); group 2: h+asb; group 3: h+0.2% chx+asb; group 4: clearfil se bond (csb); group 5: h+csb; group 6: h+0.2% chx+csb. then, composite resin rods (4×2 mm) were built up on the dentin surfaces and after thermocycling, the sbs (mpa) was evaluated. statistical analysis was performed using two-way anova and post hoc tukey tests (p < 0.05).results: there were statistically significant differences between bond strength values of group 1 (asb) and group 2 (h+asb) (p < 0.001) and group 1 (asb) and group 3 (h+chx+asb) (p < 0.001). similarly, significant differences were seen between group 4 (csb) and group 5 (h+csb) (p < 0.001) and between group 4 (csb) and group 6 (h+chx+csb) (p < 0.001).conclusion: contamination with hemostatic agent reduced the sbs of both total- and self-etching adhesive systems. in addition, application of chlorhexidine after the removal of hemostatic agent had a negative effect on sbs of total- and self-etching adhesive systems.