comparison of osteoblastic differentiation of human periodontal ligament stem cells through application of two β-tricalcium phosphate products: an in vitro study

Statement of the problem: osteoblastic differentiation of periodontal ligament stem cells (plscs) is essential for alveolar bone regeneration. purpose: the purpose of this study was to compare the potential of two β-tricalcium phos-phate (βtcp) products to induce osteoblastic differentiation of human plscs. materials and method: in this in vitro study, human plscs were cultured in mediums supplemented with guidor easy-graft [βtcp+polylactide-co-glycolide(plcg)+n-methyl-2-pyrrolidone(nmp)] [sunstar company, swiss] or sorbone [βtcp] [meta company, south korea] as two alloplasts experimental groups, mesenchymal cells differentiated into osteo-blasts without alloplast as positive control group, and mesenchymal cells without osteo-blastic induction as negative control group. osteoblastic differentiation was evaluated using alizarine red staining and spectrophotometry to assay calcium deposits and real-time poly-merase chain reaction to examine expression of alkaline phosphatase (alp) and osteopontin (opn) antigens on day 21. data were analyzed by using spss 22 software and one-way anova and bonferoni tests (p< 0.05). results: spectrophotometry confirmed that calcium deposits were higher in guidor easy-graft group compared to sorbone group (p< 0.001) and higher in two experimental groups than controls (p< 0.05). according to real-time polymerase chain reaction, level of alp expression was higher in sorbone than guidor and the levels of guidor, positive control and negative control were equal; opn levels of the positive control were more than the other groups. opn levels of sobone, guidor and negative control were the same. conclusion: these findings indicated that guidor easy-graft and sorbone enhanced differ-entiation of human plscs to osteoblasts, and could be employed as appropriate bone-graft materials.