The Impact of Calcium Hydroxide on the Osteoinductive Capacity of Demineralized Freeze-Dried Bone Allograft: an In-Vitro Study

Statement of the problem: a great challenge in periodontal therapy is the regen-eration enhancement of osseous defects through applying osteoinductive materials. demineralized freeze- dried bone allograft (dfdba) has already been introduced as an allograft with osteoconductive and variable osteoinductive properties. calci-um hydroxide [ca(oh)2] is an available well-known material in dentistry, which induces hard tissue formation. purpose: this study evaluated the efficiency of combination of dfdba and ca(oh)2 in improving the quality of osteoinduction of dfdba. materials and method: human bone marrow-derived mesenchymal stem cells were taken from volunteers’ iliac crest. cell proliferation was determined by mtt test at 18, 24 and 48 hours post-culture in 10 groups. the employed material were 0.5, 1.0 mg/ml ca(oh)2 in two forms of suspension and ph-adjusted solution, 10mg/ml dfdba per se and in combination with 0.5 and 1.0 mg/ml ca(oh)2. mineralization was assessed by alizarin red staining in 10 mg/ml dfdba, dfdba+ 0.5 and 1 mg/ml ca(oh)2 in solution and suspension forms. the data were statistically analyzed by using one-way anova and tukey’s post-hoc test (p< 0.05). results: the ph-adjusted solutions exhibited better cell proliferation compared with the suspension groups. the combination of 0.5mg/ml ca(oh)2 solution and dfdba increased the cell proliferation and mineralization compared with dfdba per se (p= 0.033). conclusion: the combination of ca(oh)2 with dfdba improved the osteoinduc-tivity of dfdba .