evaluation of the cytotoxicity effects of bacteriophage vbɸpa-1 on the virulent pseudomonas aeruginosa, a375 human skin melanoma epithelial, and hfsf pi3 human skin fibroblast cells

Background: gram-negative bacteria are the most common infectious bacteria and are life-threatening in burn patients. phage therapy studies can help the hospital community eliminate these bacterial pathogens. objectives: this study aimed to evaluate the effects of the isolated bacteriophages from hospital wastewater against gram-negative pathogenic bacteria and investigate their cytotoxicity on two human skin cell lines. methods: the bacterial strains were isolated from burn wound infections. bacteriophages were isolated from the hospital wastewater treatment plant in isfahan, iran. transmission electron microscopy, spot tests, and restriction enzyme digestion were considered for phage identification. finally, the lactate dehydrogenase (ldh) and tetrazolium-based mtt assays were used to realize the cytotoxicity impacts of the isolated phages. the statistical analysis of cell viability was performed using a two-way analysis of variance (anova). results: the results showed that among the isolated strains, p. aeruginosastrain neg_ra1300 (genbank accession number: mw845642) was sensitive to the isolated phage vbɸpa-1. the tem results showed a possible viral taxonomy of vbɸpa-1 based on its morphology, which belonged to the myoviridae (t4-like phages). the mtt and ldh experiments showed no cytotoxicity of vbɸpa-1 on two tested cell lines. conclusions: based on the results, the inhibitory effect of isolated phage on p. aeruginosaisolated from burn wounds with no toxic effect on cell lines was very significant. this phage can also be an acceptable selection against burn wound infections.