evaluating the effects of silymarin on expressing sbdsp1 and casc11 genes in hct116 colon cancer cells

Background: numerous studies have proven that silymarin can fight cancer, but the results remain controversial. objectives: this study aimed to determine the expression levels of sbdsp1 and casc11 genes in hct116 colon cancer cells to evaluate the cytotoxic effects of silymarin. methods: this study was conducted on hct 116 cell lines, which were divided into groups treated with 6.25, 12.5, 25, 50, 100, 250, and 500 g/ml silymarin and control group. the silymarin cytotoxicity was checked using the mtt assay, and the apoptosis induction rate was determined using the annexin (an)-v/pi kit and flow cytometry. the real-time pcr was also used to examine the expression of the casc11 and sbdsp1 genes. the data were compared using one-way analysis of variance between groups. results: the cell survival was reduced based on concentration and time, and ic50 concentrations were observed in 30.94, 41.50, and 46.41 g/ml at 24, 48, and 72 hours, respectively. in addition, the treatment with ic50 concentration significantly increased in apoptosis after 48 hours compared to the control. a decrease in expression of casc11 and sbdsp1 genes was observed in hct116 cells treated with ic50 concentrations after 24 and 48 hours. conclusions: according to the results, silymarin, as an active substance, declines survival, induces apoptosis, and reduces the expression of sbdsp1 and casc11 genes on the hct-116 colon cancer cell line based on concentration and time.