the regulation of testosterone levels and improvement of sperm dna maturity and in-vitro fertilization outcome by selenium administration in diazinon-treated wistar rats

Background: diazinon is an insecticide with oxidative stress properties, which is widely used in agriculture. selenium is used as a basic nutrient for animals and humans as a potential antioxidant. no studies have evaluated the protective effects of selenium on diazinon in the reproductive system of male wistar rats. objectives: the present study aimed to assess the therapeutic effects of selenium on balanced testosterone levels, sperm dna damage, fertilization, and early embryonic development in diazinontreated rats. methods: this experimental study was conducted on 32 mature male wistar rats, which were randomly assigned to four groups of eight, including control (0.2 mg/kg/b.w of corn oil), diazinon (300 mg/kg/b.w), selenium (0.5 mg of na²seo³/kg), selenium (0.5 mg/kg), and diazinon (300 mg/kg/b.w of diazinon). the animals received daily oral treatment for 60 days. results: compared to the control group, the diazinon group showed a significant reduction in the testosterone levels, sperm count, sperm motility, sperm maturation, dna maturity, invitro fertilization (ivf), twocell embryo, hatching, and blastocyst (p value < 0.05). however, the sperm mortality, sperm dna damage, and immature sperm significantly increased in the treatment group (p value < 0.05). in the diazinon and selenium treatment group, the total sperm parameters returned to the physiological range, and no significant changes were observed. conclusions: according to the results, selenium could protect the sperm parameters, testosterone level, and ivf in the diazinontreated wistar rats.