Effect of standardized polyherbal formulations on blood glucose,body weight,food and water consumption of rat

Background: standardization of polyherbal formulation is mandatory to evaluate the best quality of drugs for therapeutic value. the main objective of this study was to develop a standardized,safe and effective polyherbal juice with significant anti-oxidant and anti-diabetic activity. methods: polyherbal juice was formulated using cold maceration method and was evaluated for total phenolic content,total flavonoid content,antioxidant activity,particle size,ir,hptlc and heavy metal analysis. results: total phenolic content was found to be 139.23 ± 0.072 mg/g gallic acid equivalent and total flavonoid content was 62.01± 0.063 mg/g catechin equivalent. in dpph scavenging assay the rc50 value of polyherbal formulation was found to be 6.0 μg/ml. preliminary phytochemical tests and hptlc was performed for quantitative analysis of phytoconstituents in formulation. conclusion: in-vivo study shows significant anti-diabetic activity as compared with marketed formulations. thus polyherbal formulation possesses good anti-diabetic and antioxidant activity. © 2015 the authors.