Antibacterial Activity and Total Phenolic Content of the Onopordon acanthium L. Seeds

Background: onopordon acanthium l. is an important medicinal plant which hasbeen used in iranian medicine. in the present study, total phenolic content (tpc) wasevaluated and antibacterial properties of n-hexane and methanol extracts of o.acanthium l. seeds were screened. methods: tpc was determined by folin–ciocalteureagent method. antibacterial activities of o. acanthium were investigated using discdiffusion method, minimum inhibitory concentration (mic) and resazurin microtiterassay against some gram-positive bacteria including staphylococcus aureus,staphylococcus epidermidis, micrococcus loteus as well as gram-negative onesescherichia coli and klebsiella pneumonia. results: the tpc was found to be 168.56± 4.89 mg of gallic acid/g of extract. plant methanol extract showed significantantibacterial activity against gram-positive bacteria; staphylococcus epidermidis andmicrococcus luteus (with mic= 0.612 mg/ml), while n-hexane extract demonstratednegligible to no inhibitory activity against gram-negative and gram-positive bacteria.conclusion: the results of the present study suggested the effect of o. acanthiummethanol extract against the tested bacteria in vitro, may contribute to the in vivoefficacy of this extract