Advanced Glycation End Product Inhibitory Effect of Quercetin-3-ORutinoside,

Background: several mechanism including autoxidation of glucose, protein glycationand advanced glycation end products (ages) formation may to be involved inhyperglycemia mediated oxidative stress. herein, in this study, the effects of rutin withhigh antioxidant activity isolated from teucrium polium on structural changes ofhuman serum albumin (hsa) were performed. methods: the major compounds of t.polium methanol extract were isolated and their radical scavenging properties wereestablished through various approaches including 1,1-diphenyl-2-picrylhydrazyl(dpph), hydroxyl radicals scavenging, and reducing power. the structural changes ofhsa with glucose, in the presence of rutin were studied with far-uv cdspectroscopy. results: based on our results, rutin, a flavonol glycoside isolated fromt. polium exhibited high antioxidant activity compared to the other isolatedcompounds from t. polium. rutin inhibited high glucose induced oxidative damagesto protein by decreasing protein carbonyl formation (pco) and preventing thiols groupfrom oxidation. in addition, evaluating the structural changes of hsa by glucose inthe presence of rutin showed that rutin may be able to increase the helicity of theprotein and prevent helix decrement in the secondary structure of hsa. conclusion:we found that rutin with high antioxidant activity effectively inhibited the generationof amadori products and exhibited good inhibitory effects on the formations of ?-dicarbonyl compounds and ages.