investigation of the apoptotic effects of mesenchymal stem cells on kg-1 leukemic cell line

Background: mesenchymal stem cells (mscs) are undifferentiated cells with the ability of multi-potency, pluripotency, and self-renewal. mscs show great promise in cancer therapy due to their unique features. msc secrete various cytokines with multifunctional properties, although their roles are unclear. methods: we have investigated the influence of secreted cytokines from mscs on kg-1 cells as a cell model of acute myeloid leukemia (aml). for this purpose, following the culture and characterization of mscs, a trans-well system was used for co-culturing mscs and kg-1. to determine apoptosis induction ki/caspase-3 assay was conducted for cultured kg-1 alone and in co-culture with mscs (10:1) on day 7. in the following step, the protein was isolated from both groups (control and experimental) and western blotting was done for investigating the bax and bcl-2 proteins expression. results: it was found that mscs significantly enhanced caspase-3 activity in kg-1 cells (p<0.05). besides, a significant increase in protein expression of bax was detected, while bcl-2 displayed a dramatic reduction (p<.01). conclusion: as a concluding remark, mscs have a contributory role in the apoptosis of kg-1 cells that is mediated by caspase-3, bax, and bcl2 expression.