Mir-193a-5p Replacement Can Alter Metastasis Gene Expression in Breast Adenocarcinoma Cells in Vitro

Background: recent evidence presented the significant role of the microrna-193 (mir-193) family in biological processes by the contribution of specific targeting, which mainly display as a tumor suppressor in various cancers. in the present study, we evaluated the effect of mir-193a-5p replacement on some metastasis gene expression in metastatic breast cancer (bc) cells. methods: for this purpose, firstly, the quantitative real-time polymerase chain reaction (qrtpcr) was used to detect the mir-193a-5p expression in the mda-mb-231 bc cell line. subsequently, mir-193a-5p was transfected into the cells, and the expression levels of rock1 (rho‑associated, coiled‑coil containing protein kinase 1), cxcr4 (chemokine receptor-4), cd44, and vimentin genes were evaluated by qrt-pcr. results: the expression level of mir-193a-5p strongly reduced in mda-mb-231 cells. interestingly, the rock1 (p < 0. 001), cd44 (p < 0.0001), cxcr4 (p < 0. 001) and vimentin (p < 0. 001) expression levels significantly decreased following mir-193a-5p transfection in mda-mb-231 bc cells. conclusion: to conclude, it seems that mir-193a-5p restoration can attenuate the metastatic behavior of bc cells in vitro through decreased expression level of metastasis-related genes and may constitute an effective novel therapeutic strategy in mirna-replacement therapy and treatment of metastatic breast adenocarcinoma in the future.