green curmin reduces pro-inflammatory cytokines and fibroblast-associated colon cancer migration

Background: green curmin is a soluble curcumin extract product made in thailand that has been reported to reduce gastric inflammation. inflammatory cytokines including il-1α, il-6, and tnf-β play a crucial role in cancer cell migration, which is a major development towards poor prognosis. the aim of this study is to investigate the effects of green curmin on colon cancer cell growth and migration, which involve pro-inflammatory cytokines. methods: curcuma longa l. were extracted and named green curmin. the cytotoxicity of the green curmin-treated hct116-colon cancer cells was obtained by 3-(4,5-dimethylthiazol-2- yl)-2,5-diphenyltetrazolium bromide (mtt) assay and flow cytometry. the effect of the extract on cancer migration was investigated with a wound-healing assay. in addition, fibroblastassociated cancer migration was confirmed by using the transwell migration assay. proinflammatory cytokines were also determined. results: green curmin had cytotoxicity in the induced-cancer cell apoptosis at concentrations of 1 and 5 mg/ml. however, reduced colon cancer migration and fibroblast associated-cancer migration was found at concentrations of 0.05 and 0.1 mg/ml (non-toxicity dose), respectively. they also suppressed pro-inflammatory cytokines expression including il-1α, il-6, and tnf-β in colon cancer cells and il-6 in fibroblast cells. conclusion: green curmin has the potential to suppress pro-inflammatory cytokines and reduce fibroblast associated-cancer migration.