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prophylactic role of lactobacillus paracasei exopolysaccharides on colon cancer cells through apoptosis not ferroptosis
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نویسنده
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rahbar saadat yalda ,pourghassem gargari bahram ,shahabi arman ,nami yousef ,yari khosroushahi ahmad ,yari khosroushahi ahmad
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منبع
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pharmaceutical sciences - 2021 - دوره : 27 - شماره : 2 - صفحه:251 -261
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چکیده
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Background: nowadays despite conventional methods in colon cancer treatment, targeting vital molecular pathways and induction of various forms of cell death by safe probiotic components like exopolysaccharides (epss) are of great importance and are considered as potential therapeutic agents. this study aimed to investigate the inhibitory effect of the eps of l. paracasei on different colon cancer cell lines (sw-480, ht-29, and hct-116). methods: for this purpose, several cellular and molecular experiments including mts assay, dapi staining, annexin v/pi assay, quantitative real-time pcr (qpcr) and some important ferroptosis-related assays were performed. results: based on the findings, l. paracasei eps can induce apoptosis confirmed by all apoptosisrelated assays and could not act through ferroptosis pathways. l. paracasei eps could hinder the akt1, mtor, and jak-1 mrnas, and induces apoptosis through down-regulation of the antiapoptotic gene (bcl-2), up-regulation of pro-apoptotic genes (bax, caspase-3, 8). conclusion: the exploited eps of an indigenous probiotic strain with anticancer potential with low/insignificant cytotoxicity to normal cells is proposed for future applications in moleculartargeted therapy of colon cancer treatment. furthermore, in vivo and clinical trials should be performed to evaluate the applicability of this component besides conventional methods to increase the survival rate of colon cancer patients.
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کلیدواژه
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probiotics ,apoptosis ,exopolysaccharides ,ferroptosis ,signaling pathways ,colon cancer
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آدرس
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tabriz university of medical sciences, nutrition research center, student research committee, iran, tabriz university of medical sciences, faculty of nutrition and food sciences, nutrition research center, department of biochemistry and diet therapy, iran, tabriz university of medical sciences, faculty of advanced medical sciences, molecular medicine department, iran, agricultural biotechnology research institute of iran, branch for north-west and west region, iran, tabriz university of medical sciences, faculty of advanced medical science, drug applied research center, department of medical nanotechnology, iran, tabriz university of medical sciences, faculty of advanced medical science, drug applied research center, department of medical nanotechnology, iran
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پست الکترونیکی
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yarikhosroushahia@tbzmed.ac.ir; ayarikh@gmail.com
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Authors
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