>
Fa   |   Ar   |   En
   protective effect of merbau (intsia bijuga) extract on hydrogen peroxide-treated hacat human keratinocytes and its formulation as antioxidant cream  
   
نویسنده hartiadi leonny yulita ,sahamastuti agnes anania triavika ,chandra cynthia valentina ,febriani erlina ,adiyanto shakila angjaya ,daeli giovanni batista christian ,clarissa gabriella cindy
منبع pharmaceutical sciences - 2021 - دوره : 27 - شماره : 1 - صفحه:131 -138
چکیده    Background: the excessive generation of ros in the skin results in oxidative stress that can contribute to premature skin aging, inflammation, and skin carcinogenesis. to prevent these detrimental effects, the development of herbal medicine with a potent antioxidant activity into cosmetic products is required. this study aims to formulate cream that contains a safe and effective concentration of merbau (intsia bijuga), which has been shown to have a strong antioxidant activity. methods: powdered merbau wood was macerated with methanol and the dried extract was evaluated for its cytotoxic effect and antioxidant activity on human keratinocytes cell line using mts assay. five cream formulations containing the extract were made and subjected to stability and physical evaluations, including organoleptic, types of cream, ph, viscosity, and homogeneity. results: cytotoxicity assay revealed that merbau extracts had an ic50 of 181.3 μg/ml (95% confidence interval (ci): 165.4 - 200.1 µg/ml). at a concentration of 31.25 μg/ml, the extract exhibited a protective effect against h2 o2 -induced oxidative stress, comparable to vitamin e. five cream formulas that were developed demonstrated good physical properties that fulfilled the evaluation parameters, including o/w type of cream, homogenous, and stable based on centrifugation and freeze-thaw cycle tests. the ph values were between 5.65 ± 0.067 - 7.4 ± 0.050, while the viscosity values were between 131 ± 1.249 - 56,011 ± 2,729.27 mpa.s. all cream formulas exhibited shear-thinning properties upon increasing shear stress. conclusion: overall, this study has successfully formulated several cream formulations containing merbau extract at a concentration that shows antioxidant activity.
کلیدواژه antioxidant ,cream ,human keratinocytes ,intsia bijuga ,merbau
آدرس indonesia international institute for life sciences, faculty of life sciences, pharmacy study program, indonesia, indonesia international institute for life sciences, faculty of life sciences, pharmacy study program, indonesia, indonesia international institute for life sciences, faculty of life sciences, biomedicine study program, indonesia, indonesia international institute for life sciences, faculty of life sciences, biomedicine study program, indonesia, indonesia international institute for life sciences, faculty of life sciences, pharmacy study program, indonesia, indonesia international institute for life sciences, faculty of life sciences, pharmacy study program, indonesia, indonesia international institute for life sciences, faculty of life sciences, biomedicine study program, indonesia
پست الکترونیکی gabriella.cindy@student.i3l.ac.id
 
     
   
Authors
  
 
 

Copyright 2023
Islamic World Science Citation Center
All Rights Reserved