development of salt induced liquid–liquid extraction combined with amine based deep eutectic solvent-dispersive liquid–liquid microextraction; an efficient analytical method for determination of three anti-seizures in urine samples

Background: a simple, fast, efficient, and environmentally friendly microextraction technique named salt induced liquid-liquid extraction combined with amine based deep eutectic solventdispersive liquid-liquid microextraction method followed by gas chromatography-flame ionization detector was applied for the determination of some anti-seizure drugs in urine samples. methods: in this method, sodium chloride (30% w/v) was dissolved in the sample solution (adjusted at ph=10) as phase separation agent and iso–propanol was added to the solution. after manually shaking, the solution was centrifuged and the supernatant phase was removed and mixed with choline chloride: benzyl ethylenediamine (85 μl) and the mixture was rapidly dispersed into a deionized water containing 5.0 % w/v, sodium chloride and adjusted at ph=10. the cloudy solution was centrifuged and the sedimented phase was removed and 1 μl of it was injected into the determination system. results: under final conditions, the limits of detection and quantification at the ranges of 3.4-6.9, and 11.5-23.3 ng ml^-1 were obtained, respectively. the relative standard deviations for intraand inter day assays were lower than 10.9%. the effect of exogenous and endogenous effect on the performance of the method was studied and the results showed that these factors were nearly effect less on the efficiency. conclusion: the introduced method was satisfactorily utilized to determinate the selected benzodiazepines drugs in the patients’ urine samples.