simultaneous determination of phenobarbital, phenytoin, carbamazepine and carbamazepine-10,11-epoxide in plasma of epileptic patients

Background: quantitative analyses of antiepileptic drugs are required in clinic and to rational dosage adjustment, the clinician needs the blood levels of these drugs. a high-performance liquid chromatography with spectrophotometric detection has been developed and validated for simultaneous determination of some antiepileptic drugs in plasma of patients with epilepsy. methods: a simple procedure based on deproteinization by acetonitrile was used for pre-treatment of plasma samples. liquid chromatographic analysis was carried out on a nova-pak® c18 analytical column, using a ternary mixture of potassium dihydrogen phosphate buffer (ph 6.0)-acetonitrile-2-propanol (63:22:15, v/v/v) as the mobile phase, at a flow rate of 1.0 ml min^-1. results: calibration curves were linear over a range of 1–40 μg ml^-1 for phenobarbital, 1–30 μg ml^-1 for phenytoin, 0.3–15 μg ml^-1 for carbamazepine and 0.5–6 μg ml^-1 for carbamazepine epoxide. conclusion: the simple sample pre-treatment, combined with the fast chromatographic run was used for the determination of antiepileptic drugs for a large number of patient samples.