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chromatographic method for determination of the amino acid content in dioscorea bulbifera l. tubers by rp-hplc
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نویسنده
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bhat musadiq hussain ,fayaz mufida ,kumar amit ,dar alamgir ahmad ,jain ashok kumar
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منبع
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pharmaceutical sciences - 2019 - دوره : 25 - شماره : 1 - صفحه:65 -69
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چکیده
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Background: the present study was carried out for determination of amino acid content in tubers of dioscorea bulbifera using reverse-phase high-performance liquid chromatography. methods: the method involved the vapor phase hydrolysis of the sample, automated derivatisation of the amino acids with the aid of accq-fluor reagent kit, separated on a high performance liquid chromatography equipped with photo diode array (hplc-pda) at 254 nm having column temperature of 37 °c. results: the proportional molar concentration for each amino acid was calculated based on the concentration of standard amino acids and expressed as μg amino acid/mg sample. methionine, aspartic acid and leucine were major components while as tyrosine was found minor from the plant on dry weight basis. conclusion: the method is reliable, simple and economical for determining the amino acid content of dioscorea bulbifera tubers.
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کلیدواژه
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dioscorea bulbifera ,amino acids ,high performance liquid chromatography ,accqfluor
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آدرس
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jiwaji university, school of studies in botany, india, jiwaji university, school of studies in botany, india, jiwaji university, institute of ethnobiology, india, sher‐e‐kashmir university of agricultural sciences and technology (skuast‐k), research centre for residue and quality analysis, india, jiwaji university, institute of ethnobiology, india
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پست الکترونیکی
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tclabju17@gmail.com
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Authors
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