development of a micellar electrokinetic chromatographic method with indirect uv detection for pregabalin determination in serum samples

Background: a micellar electrokinetic chromatographic (mekc)/ indirect uv detection method with hydrodynamic and electrokinetic injection has been developed for the determination of pregabalin in the serum samples. methods: separation of the drug was achieved on agilent capillary electrophorese in less than 5 min using a 50 cm × 75 μm i.d. uncoated fused-silica capillary and a background electrolyte (bge) consisting of 5-aminosalicylic acid (5-asa, 10 mmol l^-1), cetyl trimethylammonium bromide (ctab, 1 mmol l^-1) and tri-sodium citrate (4% w/v). the influence of various parameters on the separation such as separation voltage, injection time, cassette temperature, ph of bge and organic modifier was investigated. results: method validation shown good linearity (r²> 0.999) in the range of 1.5-100 μg ml^-1 of pregabalin. a limit of detection (lod) of 0.8 μg ml^-1 and a limit of quantitation (loq) of 2.6 μg ml^-1 were reported for pregabalin. conclusion: the proposed method was found to be suitable and accurate for the determination of pregabalin in serum samples.