ارزیابی سازگاری پلاکتی بین بیماران aml و اهداکنندگان پلاکت به روش فلوسیتومتری

Abstractbackground and objectivesone of the complications of repeated platelet transfusion is immune platelet refractoriness. in this study, a flow cytometic platelet cross-matching was performed to evaluate platelet transfusion outcome.materials and methodsin this descriptive study, fifteen patients with a history of multiple platelet transfusions and fifteen healthy participants as the control group were enrolled in this study. platelet cross-match was done and analyzed by the gate method. edta-anticoagulated blood samples from healthy donors were collected and prp sampls were obtained. the serum of each patient was added to platelet suspension and incubated with fitc-anti human igg and was analyzed using a flow cytometer. platelet cross-match results of the patients were compared with those of cci.resultsplatelet cross match results were negatively correlated with 1-hour (r = -0.731, p = 0.002) and 24-hour (r = -0.794, p = 0.001) ccis. there was a significant difference in the mean percentage of platelet crossmatch between the patients with immune platelet refractoriness (showing 1-hour ccis less than 7500) and that of the control group and the patients without platelet refractoriness.conclusionsthe results showed that there was a significant correlation between platelet cross match by flowcytometry and 1-hour cci results and this method could be applicable in the evaluation of platelet transfusion outcome.