گسترش سلول‌های پیش‌ساز خونساز بند ناف انسانی بر روی داربست 3 بعدی پوشیده شده با نانوالیاف کلاژنی

Abstractbackground and objectivesthe allogeneic hematopoietic stem cell transplantation is used for the treatment of some hematological and non-hematological disorders, but the lack of suitable donors for many patients limits the use of this source therapy. umbilical cord blood (ucb) is an alternative source of hematopoietic stem cell (hsc) transplantation. despite all advantage lower cell doses is major obstacle in cord blood transplantation. exvivo expansion of hsc is an alternative way to overcome this problem. materials and methodsin this experimental study, polycaprolactone (pcl) scaffold coated with nano fibrous collagen compared to routine cell culture system is used for cell culture. total cells, cd34 cell, cfc assay, and cxcr-4 expression was performed.resultsour findings demonstrated that the total cell expansion and cd34+ cells in 3 dimensional (3d) scaffold compared to routine culture system (2d) was higher (p < 0.05). also total colony cells in 3d scaffold was higher than 2d cell culture system and statically significant (p < 0.05). higher cxcr-4 expression in 3d compared to 2d showed better homing of cells cultured in 3d scaffold (p < 0.05).conclusionspcl scaffold coated with nano fibrous collagen was a proper cell culture system compared to 2d for cell expansion.