اثر اپی‌نفرین بر بیان ژن CXCR4 در سلول‌های بنیادی خونساز CD34+ خون بند ناف

Abstractbackground and objectivesrecently, circulating hematopoietic stem cells (hsc) have been used for the treatment of most types of leukemia. however, the g-csf mechanism has not been known yet but it is believed that g-csf is mostly effective by its indirect functions. the suppression of the nervous system affects g-csf induced mobilization of hsc. the main gene involved in mobilization is cxcr4 that ligand to sdf-1. so, this study investigates the effect of the main catecholamine of nervous system-epinephrin- on cxcr4 expression.materials and methodsin this experimental study, isolated cord blood cd34+ cell of 20 healthy newborn with macs columns treated with 10 ?m epinephrine and 1 ?m proporanolol. expression of cxcr4 has been investigated at 1, 3 and 5 hours with qrt-pcr. receptors of beta adrenergic expression have been studied with rt-pcr. resultsbeta adrenergic receptors are expressed on cd34+ cells. epinephrine led to significantly increased cxcr4 in hours 1 (3.2 ± 0.5), 3 (2.4 ± 0.4) and 5 (2.2 ± 0.4 ). cells treated with propranolol, return increased cxcr4 induced by epinephrine.conclusionsit can be concluded that g-csf affects the expression of cxcr4 through increasing secretion of epinephrine in bone marrow with on beta adrenergic receptors. cells with increased cxcr4 mobilized to release sdf-1 in peripheral blood.