جداسازی و تعیین هویت سلول‌های بنیادی خونساز و مزانشیمی مشتق از بافت جفت انسانی

Abstractbackground and objectivesthe low frequency of hematopoietic stem cells in umbilical cord blood is the main factor of its application to be restricted for transplantation. if hematopoietic and mesenchymal stem cells of placenta can be isolated and transplanted with cord blood cells, the problem of cell dose limitation will be solved due to increase in the number of primary hematopoietic stem cells and homing of cells through mesenchymal stem cells. thus in this study, we tried to isolate the maximum number of hematopoietic and mesenchymal stem cells from placenta by enzymatic methods and evaluate their properties.materials and methodsfive different placenta tissues were treated with collagenase and their red blood cells were omitted with the lysis buffer; then, the remaining cells were studied for the presence of hematopoietic and mesenchymal stem cells.resultsthe result showed that 6.02 ± 0.6 and 4.78 ± 0.8 percent of isolated cells were cd34+ and cd34+ together with cd38-, respectively. these cells having generated all hematopoietic lineages and adherent stromal cells with the property of mesenchymal stem cells were obtained from the culture of placenta cells.conclusions the presence of a remarkable number of cd34+ cells and mesenchymal stem cells in placenta tissue showed that transplantation of these cells with umbilical cord blood cells can increase the quality of transplantation.