مطالعه پتانسیل تکثیری و تمایزی سلول‌های تک هسته‌ای خون بند ناف تکثیر شده در بیوراکتور سوسپانسیونی مجهز به هم‌زن رفت و برگشتی

Abstractbackground and objectivesumblical cord blood (ucb) hematopoietic stem cells having high cell counts are suitable candidates for benign and malignant disease transplantation but often ucbs have low counts. in this study, ex vivo expansion of these cells was performed using suspension bioreactor with vertical agitation. materials and methodsucb mononuclear cells were cultured with 1.07 × 106 cells/ml density in total 206 ml of culture medium in the suspension bioreactor with vertical agitator; and the static group with same density was cultured in t-flasks for 14 days. at the days 0, 3, 7 and 14 of culture, sampling was performed for further analysis.resultsduring the 3 days of culture, differentiated cells were lost and then the number of cells increased again from 0.42 × 106 cells/ml up to 1.2 × 106 cells/ml and from 0.6 × 106 up to 2.9 × 106 in bioreactor and static culture, respectively. the percentage of cd34+ cells and colony forming potential significantly increased in bioreactor.conclusions hematopoietic stem cells in the bioreactor while retaining their self-potential are expanded. however, due to the increasing shear stress in the environment, the total number of cells did not increase. therefore, further studies are required.key words: cord blood, hematopoietic stem cells, bioreactors