اثر نانوفیبر پلی اتر سولفان آمینه بر افزایش تکثیر و کاهش آپوپتوزیس سلول‌های خونساز خون بند ناف

Abstractbackground and objectivesumbilical cord blood is a promising alternative source of hematopoietic stem/progenitor cells (hspcs) for allogeneic hematopoietic stem cell transplantation. although the use of ucb for transplantation has plentiful advantages in comparison with other cell sources, the low number of hspcs led to cell delayed recovery. therefore, ex vivo expansion of hspc before transfusion could induce faster cell recovery. materials and methodscd34 positive cells were positively enriched using midimacs system and cultured on aminated ps nanofibers, aligned pcls, and random pcls. cell expansion and quantification of cell apoptosis were carried out in expanded cell populations in different conditions by the flowcytometric method. resultsmicroscopic and flowcytometric analysis revealed the differentiation of cd133+ cord blood hematopoietic stem cells to erythroid lineage. flow cytometry showed that both of the ppar? agonists were able to diminish transferrin receptor and glycophorin a positive cell population significantly. the inhibitory effect of ppar? agonists on erythroid differentiation was dose-dependent. the inhibitory effect of troglitazone on colonies formation of erythroid was markedly higher than bicaline.conclusions this study demonstrated that aminated ps nanofibers has positive effects on the expansion of cd34+ cells compared to 2d culture system by increasing cell expansion and cell apopotosis inhibition. however, aligned and random pcl nanofibers did not show supportive effects on cell proliferation.