اثر داربست «آلوگرافت استخوانی معدنی شده» با پوششی از سلول‌های استرومال خون بند ناف، بر تکثیر برون تن سلول‌های بنیادی خونساز CD34+

Abstractbackground and objectivesbone marrow transplantation with umbilical cord blood (ucb) in adult recipients is limited mainly by a low cd34+ cell dose. to overcome this shortcoming by minimum manipulation, mineralized bone allograft (mba) scaffold coated by ucb-ussc (unrestricted somatic stem cells) was used to expand cd34+ cells from ucb. materials and methodsin this experimental study, ucb-usscs were isolated and characterized by morphologic and immunophenotypical analysis. then they were seeded on mba (for 3d figuration) and culture plate (for 2d figuration) as a feeder layer. cd34+ cells were isolated from the ucb (by macs method) and were expanded in 2d and 3d conditions for 3 weeks. at the end, cell count, flow cytometry, cologenic assay, and ltc-ic were done.resultsafter 3 weeks, ex vivo expansion of ucb-cd34+ was enhanced 250 ± 13.2 in 3d. the highest cfc expansion and ltc-ic were observed at day 14. flow cytometry analysis showed the lowest percentage in 3d culture.conclusionsusscs by 3d mba structure produce the hematopoietic cytokine, extracellular matrix and binding molecules; they also create the intercellular interaction that can be used as a suitable feeder layer. it provides an ex vivo mimicry of bone marrow niche by enhancing surface/volume ratio. this model could be a suitable and alternative environment for hscs expansion and hematopoiesis.