تاثیر miR-146a و miR-150 بر القای تمایز سلول‌های لنفوییدی T در سلول‌های بنیادی خونساز

Abstractbackground and objectivesmicro rnas are a class of small non-coding rnas which have been recently shown to play a crucial role in major cellular processes such as development and differentiation through post-transcriptional regulation. the role of these epigenetic elements has been also demonstrated in hematopoietic lineage differentiation and there is a large body of evidence that mir-150 and mir-146a are responsible for t lymphocyte differentiation. our goal was to examine the effect of mir-150 and mir-146a overexpression in hematopoietic stem cells and its ability to differentiate these cells into a t lymphoid cell. materials and methodsin this experimental study cd133+ stem cells were employed as hematopoietic stem cells and permanent overexpression of mir-150 and mir-146a was established. the differentiation progress was tracked by flowcytometry for lymphocyte markers such as cd4 and cd8. moreover, expression of mir-146a and mir-150 by rt-pcr and quantitative real time pcr after 7 days was studied. resultsthe cells showed t lymphoid characteristics 7 days after transduction. cd4 and especially cd8 expression significantly increased.conclusionsin conclusion, mir-150 and mir-146a overexpression is an effective factor in t cytotoxic differentiation and they have the ability of directing cd133+ hematopoietic stem cells to express t lymphoid characteristics.