Evaluation the Efficacy of HBA Sperm-Hyaluronan Binding Assay Method in Infertility Centers

Introduction: the novel development of the (hba) sperm- hyaluronan binding assay hasnow been routinely used in some laboratories worldwide to predict sperm maturity andfunctionality. the purpose of this study was to evaluate the correlation between hba score andhistone deficiency, protamine content and dna fragmentation.methods: during april- march 2012 patients who were enrolled at shiraz- human assistedreproductive center were included in this study. semen sample from 100 men were assessedduring this study. patients were divided into two groups, group a (%15≤hba≤%55) n = 50and group b (hba≥ %55) n = 50. sperm analysis carried out according to world healthorganization criteria. histon efficiency, protamine content, and dna integrity were assessed byaniline blue, chromomycine a3 staining and sperm chromatin dispersion test.results: the results show positive correlation between sperm concentration and motilitywith percentage ha-bounded sperms while a negative correlation with percentage abnormalmorphology. histone deficiency assessed using aniline blue staining show significant differencein group a 18.22±1.06 compared with group b 32.43± 2.90. furthermore, the percentage ha-bounded sperm showed a significant negative correlation with protamine deficiency assessedby cma3 staining spermatozoa (15.06± 0.88 in group a versus. 75.17± 2.37 in group b). theresults also show a positive correlation between intact dna with ha bound ability.conclusion: the results indicating that hba had a high degree of selectivity for sperm withhistone efficiency, protamine quality, and high dna integrity. these findings are important fromthe points of view of human sperm dna integrity, sperm function, and the potential efficacy ofha-mediated sperm selection for human assisted reproduction techniques.