Effect of Olive Leaf Extract in Inhibition of Renal Ischemia-Reperfusion Injuries in Rat

Introduction: renal ischemia reperfusion is considered to be one of the causes of acuterenal failure (aki). in addition, renal ischemia is an important incident usually occurs due torenal transplantion, repair of suprarenal aneurysm, hemorrhagic shock. a recent study reportedthat reactive oxygen species (ros) are important mediators of aki. it seems essential to useantioxidant to decrease the effect of free radicals. the olive leaf extract (ole) has an abundantantioxidant characteristic the purpose of this study was to investigate the effects of olive leafextract on the control of renal injuries caused by renal ischemia-reperfusion in an animal model.methods: forty male sprague-dawley rats were randomly divided into 5 equal groups: control,i/r+ 0.5 ml water by gavages, i/r+ole in 3 different doses ( 25mg/kg, 50mg/kg, 100mg/kg) once daily for 2 week, prior to i/r. the right and left pedicles clamped for 45 minutes(ischemia), followed by 24 hours of reperfusion. serum bun and creatinine were measured,and serum malondialdehyde (mda) was determined, renal histologically parameters such astubular necrosis, eosinophilic casts were studied semi quantitatively and tubular dilatation wasmeasured by motic image plus 2 software, and renal antioxidant enzyme activity such asglutathione was assessed.results: i/r group significantly increased serum cr and bun, serum mda, and tubularnecrosis, casts, tubular dilatation, and significantly decreased glutathione compared with thecontrol group. pretreatment with ole+i/r in 3 different doses significantly decreased tubulardilatation, tubular necrosis and casts, serum mda, and increased glutathione comparedwiththe i/r group. but only 4 group significantly decreased serum cr and bun compared with thei/r groupconclusion: the findings showed that two week pretreatment of rats with ole by gavages inits three doses (25mg/kg, 50mg/kg and 100mg/kg) can ameliorate renal injuries compared withi/r group. in order to consider the effects of all antioxidant of ole in decreasing caused by i/rfurther researches recommended.