In Vitro Study of the Protective Effects of Hydroalcholic Extract of Soybean against Impact of Oxidative Damage on Osteogenesis and Chondrogenesis of Mouse Limb Bud

Introduction: oxidative stress has been implicated in the pathogenesis of various diseasesaffecting chondrogenesis or the function of articular cartilage. the purpose of the present studywas to find the effect of soybean extract on reduction of detoriation effects of oxidative stress inembryonic chondrogenesis in vitro.methods: in order to separate ectoderm from mesenchyme, the limb buds of mouse embryos (12-13days) removed and incubated in dispase. after separation, the limb bud rinsed and incubated inthe trypsin. adding culture media (dmem/f12 contain 10% fbs) and pipetting, the limb budswere dissociated. the number of the cells adjusted to 1-2 ×107/ml. superconfluent micro massspotting of cell suspension in 96 well tissue culture plate were formed. after incubation for 1.5-2h at 37 ºc and 5% co2, the plate flooded with culture media. at day 5, different concentration ofh2o2 and soybean extract added to fresh media and in order to induce oxidative stress, incubatedfor 24h. the cultures were stained with alcian blue to prove the cartilage differentiation andalizarin red for calcium deposit.results: the results indicated that cell viability diminished by extract and h2o2 administration;although, the supplementation of the cells exposed to the oxidative stress with the extractimproved cell proliferation rate. the soybean also improved the ossification and chondrificationof the cells exposed to h2o2.conclusion: the study demonstrated that soybean extract can reduce the effect of oxidativestress in embryonic chondrogenesis.