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Background and Objectives: Mouthwashes are commonly used to reduce the harmful effects of oral bacteria. The purpose of the present study was to compare the antibacterial effects of Matrica and Chlorhexidine 0.2% mouthwashes on common microorganisms in periodontal diseases.Materials and Methods: This is a laboratory study in which disc diffusion method was used. The diameter of the inhibition zone caused by the antimicrobial effect of Matrica and Chlorhexidine 0.2% was measured in millimeter. Data were analyzed using independent ttest and oneway ANOVA.Results: The highest mean diameter of the inhibition zone caused by Matrica was 14.85 ±0.42 and in case of Chlorhexidine 0.2%, 25.54 ±0.49. Chlorhexidine showed more inhibitory effect compared to Matrica (p˂ 0.001).Conclusion: The results of this study showed that chlorhexidine compared to Matrica has a more antibacterial effect on bacteria that are effective in periodontal diseases. Matrica mouthwash has a more inhibitory effect on the Actinomyces viscosus compared with the other two bacteria.Key words: Antiinfective agents, Chlorhexidine, Matrica, Streptococcus mutans, Actinomyces viscosus, Enterobacter cloacae Funding: This research was funded by Rafsanjan University of Medical Sciences.Conflict of interest: None declared.Ethical approval: None declared.How to cite this article: Salari Sedigh S, Sadeghi M, Beikizadeh M, Rezazadeh Zarandi E, Assar Sh. Comparison of Antimicrobial Effects of Matrica Herbal Mouthwash and 0.2% Chlorhexidine on the Growth of Streptococcus Mutans, Actinomyces Viscosus, and Enterobacter Cloacae: A Short Report. J Rafsanjan Univ Med Sci 2019; 18 (3): 30512. [Farsi]