the effect of eight weeks of high-intensity interval training on the expression of mir-126, emap-ii, and levels of tgf-β in the heart tissue of young rats with type 2 diabetes

Background and aims: diabetes affects some cardiac angiogenic and antiangiogenic markers. thus, the aim of this study was to investigate the effect of eight weeks of high-intensity interval training (hiit) on mir-126, endothelial monocyte-activating polypeptide ii (emap-ii), and transforming growth factor beta (tgf-β) in young rats with type 2 diabetes (t2d). methods: the statistical population of this experimental study included young rats (8 weeks old) with t2d. in this research, 24 young rats (8 weeks old) were divided into healthy+hiit, diabetic+hiit, and healthy control groups. the enzyme-linked immunosorbent assay method was used to measure tgf-β and the real-time polymerase chain reaction for mir-126 and emap-ii. a one-way analysis of variance and the least significant difference post hoc test were used to compare the two groups. results: the results showed diabetes+hiit and healthy+hiit had more mir-126 compared to healthy control (p=0.001). healthy+hiit, in comparison to diabetes+hiit, had higher mir-126 expression (p=0.001). in addition, diabetes+hiit, compared to the healthy control, had a higher emap-ii expression (p=0.006). on the other hand, healthy+hiit had lower emap-ii in comparison to healthy control (p=0.001). healthy+hiit had a lower emap-ii compared to diabetes+hiit (p=0.001). diabetes+hiit, in comparison to the healthy control, had higher tgf-β levels (p=0.005). however, healthy+hiit, compared to healthy control, had a lower expression of tgf-β (p=0.001). finally, the healthy+hiit groups, compared to the diabetes+hiit group, had a lower tgf-β expression (p=0.001). conclusion: interval exercise could increase angiogenesis in diabetic samples through the increase of mir-126 and the reduction of tgf-β1 and emap-ii.