the effect of high-intensity interval training with caloric restriction on gene expression of apoptosis indices hsp70 and hsp60 in the myocardium of male rats

Background and aims: the purpose of this study was to investigate the effect of high-intensity interval training combined with calorie restriction on the expression of hsp70 and hsp60 in the myocardium of male rats. methods: a total of 27 male rats were randomly divided into three groups (9 rats in each group): control, caloric restriction, and caloric restriction with high-intensity interval training. during the study, the control group had free access to water and food, but the amount of food in the food restriction group was limited to 50% and the calorie restriction-exercise group was limited to 25% of the control group. the restriction-exercise group was subjected to intense interval training for eight weeks. the gene expression of apoptotic indicators hsp70 and hsp60 was assessed using the real-time pcr method. the data were analyzed using shapiro- wilk test and anova test. results: body weight in both calorie restriction groups significantly decreased (p = 0.001). the results of the post hoc test showed a significant increase in the expression of hsp70 and hsp60 genes in the myocardium of male rats in the calorie-restricted-exercise group compared to the calorie-restricted and control groups (p = 0.001). additionally, no significant difference was observed between the two restriction and control groups in terms of hsp70 and hsp60 (p = 0.102). conclusion: the combination of high-intensity interval training and mild food restriction significantly increased hsp60 and hsp70 expression.