the effect of aerobic training and eugenol supplementation on the pi3k/akt/mtor pathway in skeletal muscle of male rats poisoned with chlorpyrifos

Background and aims: chlorpyrifos (cpf) is an insecticide that is widely used in the world. the purpose of this research was to investigate the effect of 4-week aerobic exercise and eugenol supplementation on the phosphatidylinositol-3-kinases/protein kinase/mammalian target of rapamycin (pi3k/akt/mtor) pathway on the skeletal muscle of male rats poisoned with cpf. methods: overall, 12-week-old female rats were used in this experimental research. the rats were randomly divided into 8 groups (8 rats in each group), including healthy control, toxic control, poison solvent, corn oil solvent, poisoned+eugenol, poisoned+aerobic exercise, and poisoned+aerobic exercise+eugenol. moderate training was in the range of 50-60% vo2 max, including 5 training sessions per week (treadmill). poisoning was performed with cpf poison with a dose of 3 mg/kg, and the dose of eugenol was determined to be 250 mg/kg. results: there was no significant difference between the groups in terms of mtor and akt expression (p=0.369, p=0.59). however, the expression of pi3k in the poisoned control group was lower than that in the healthy control group (p=0.049). in addition, the expression of pi3k was higher in the poisoned+eugenol+exercise group compared to the poisoned control group (p=0.009). the corn solvent group also had a higher pi3k expression in comparison to the poisoned control group (p=0.025). finally, there was no significant difference among the other groups. conclusion: in general, 4 weeks of cpf poisoning caused a significant decrease in pi3k, but it did not have a significant effect on akt and mtor. based on the finding, 4 aerobic exercises and eugenol consumption could significantly increase in pi3k, while it had no significant effect on akt and mtor.