the effect of eight weeks of high-intensity interval training and moderate-intensity continuous training on some factors causing oxidative stress in the cardiomyocytes of mice with type ii diabetes

Background and aims: diabetic cardiomyopathy (dcm) is a major cause of morbidity and mortality among diabetic patients. this study aimed to compare and investigate the effects of high-intensity interval training (hiit) and moderate-intensity continuous training (mict) on oxidative stress as one of the key links in the development of dcm in mice with type 2 diabetes mellitus (t2dm). methods: forty male c57bl/6j mice were randomly assigned to four groups of equal number (control, t2dm, t2dm+hiit, and t2dm+mict). after the induction of t2dm, hiit and mict programs were conducted 5 days a week for 8 weeks. at the end of the experimental period, blood and heart samples were collected for subsequent measurements. results: t2dm significantly up-regulated the levels of advanced glycation end products (ages, p=0.001) and the expression of 15-lipoxygenase (15-lox, p=0.001) and 4-hydroxy-2-nonenal (4-hne, p=0.001) compared to controlled mice. after 8 weeks of training, hiit and mict programs increased the levels of insulin (p<0.001) while reducing the levels of fasting blood glucose (p<0.001, p=0.024, respectively). on the other hand, it was found that hiit and mict programs significantly decreased the levels of ages (p<0.001), expression of 15-lox (p=0.006, p=0.019, respectively), and 4-hne (p=0.008 and p=0.035, respectively). conclusion: our findings revealed that exercise training, particularly mict, will be highly helpful in the prevention of dcm through the improvement of glucose metabolism and reduction of 15-lox expression.