the effect of aerobic exercise training on gene expression of beta3-adrenergic receptor and beta-arrestin2 in inguinal white adipose tissue of mice fed with a high fat diet

Background and aims: beta-adrenergic signaling deficiency has been established to be related to obesity and related diseases. beta3- adrenergic receptor (adrb3) and beta-arrestin2 (barr2) are pivotal agents in the beta-adrenergic-signaling pathway. this study aimed to investigate the preventive effect of aerobic training on dysregulation of adrb3 and barr2 gene expression that was induced by high-fat diet (hfd) in inguinal white adipose tissue of mice. materials and methods: twenty-one c57bl/6 mice were assigned to three groups as follows: 1) control group (n=7), 2) high-fat dietinduced overweight (hfd-ow) (n=7), and 3) high-fat diet with aerobic training (hfd-at) (n=7). the hfd-ow group were fed with a hfd for 12 weeks. the hfd-at group had aerobic training for six weeks on a treadmill in addition to feeding with the hfd. the real-time polymerase chain reaction (pcr) method was used to measure the gene expression of adrb3 and barr2 in inguinal white adipose tissue. results: the gene expression of adrb3 did not significantly change between groups (p > 0.05). however, the expression of barr2 in hfdow group was significantly increased as compared to the control group (1.5-fold: p = 0.001). interestingly, the barr2 expression in hfd-at group was significantly lower compared with hfd-ow group (p = 0.045). conclusion: the results indicated that aerobic training could inhibit the upregulation of barr2 induced by hfd. it seems that a portion of the preventive effect of aerobic training on the development of obesity may be mediated by inhibiting the barr2 expression in adipose tissue.