short-term exercise training increases plasma levels of klotho and total antioxidant capacity in male wistar rats

Background and aims: soluble or circulating form of klotho (i.e., antiaging and longevity protein) has biological effects on various body cells and tissues. considering the role of exercise training on protective proteins, the present study aimed to investigate and compare the effects of shortterm highintensity interval training (hiit) and moderateintensity continuous training (mict) on plasma levels of klotho, total antioxidant capacity (tac), and malondialdehyde (mda). methods: in this study, 24 male wistar rats with a weight range of 250-300 g and 8-10 weeks old were randomly divided into control, hiit, and mict groups. training included five consecutive days on the treadmill. hiit including 6×2 minutes high intermittence with 85%-90% vo2 max and 5×2 minutes slow intermittence (active recovery) with 55%-60% vo2 max. mict program was performed at 70% vo2 max that total distance running was the same for the hiit group. then, the plasma levels of klotho, tac, and mda were measured by elisa assay. finally, oneway anova and posthoc tukey tests were used for statistical analysis. results: based on the results, a significant increase was observed in plasma levels of klotho and tac in both hiit and mict groups compared to the control group (p<0.001) while these changes were significantly more dominant in the hiit group compared to mict group (p=0.024). however, the results demonstrated no significant difference between the groups regarding mda (p=0.109). contrarily, the finding revealed a significant positive correlation between klotho and tac (r=0.79, 0.83, 0.79 in control, hiit, and mict groups, respectively, p<0.05), whereas a significant negative association between klotho with mda within all the groups (r= 0.78, 0.86, and 0.81). conclusion: in general, even shortterm aerobic exercise training, especially hiit increases circulating klotho and tac, therefore, this can confirm the positive effects of the traininginduced exercise.

Shortterm exercise training increases plasma levels of klotho and total antioxidant capacity in male Wistar rats

Background and aims: Soluble or circulating form of Klotho (i.e., antiaging and longevity protein) has biological effects on various body cells and tissues. Considering the role of exercise training on protective proteins, the present study aimed to investigate and compare the effects of shortterm highintensity interval training (HIIT) and moderateintensity continuous training (MICT) on plasma levels of Klotho, total antioxidant capacity (TAC), and malondialdehyde (MDA). Methods: In this study, 24 male Wistar rats with a weight range of 250300 g and 810 weeks old were randomly divided into control, HIIT, and MICT groups. Training included five consecutive days on the treadmill. HIIT including 6×2 minutes high intermittence with 85%90% Vo2 max and 5×2 minutes slow intermittence (active recovery) with 55%60% Vo2 max. MICT program was performed at 70% Vo2 max that total distance running was the same for the HIIT group. Then, the plasma levels of Klotho, TAC, and MDA were measured by ELISA assay. Finally, oneway ANOVA and posthoc Tukey tests were used for statistical analysis. Results: Based on the results, a significant increase was observed in plasma levels of Klotho and TAC in both HIIT and MICT groups compared to the control group (P<0.001) while these changes were significantly more dominant in the HIIT group compared to MICT group (P=0.024). However, the results demonstrated no significant difference between the groups regarding MDA (P=0.109). Contrarily, the finding revealed a significant positive correlation between Klotho and TAC (r=0.79, 0.83, 0.79 in control, HIIT, and MICT groups, respectively, P<0.05), whereas a significant negative association between Klotho with MDA within all the groups (r= 0.78, 0.86, and 0.81). Conclusion: In general, even shortterm aerobic exercise training, especially HIIT increases circulating Klotho and TAC, therefore, this can confirm the positive effects of the traininginduced exercise.