مطالعه اثر محافظتی نانوکپسول سیاه‌دانه بر آسیب‌شناسی بافتی و سطوح اینترلوکین 10 بافت ریه رت‌های در معرض Nnk

اهداف: این فرض وجود دارد که نیتروزآمین کتون مشتق از تنباکو (nnk) که مهم ترین کارسینوژن موجود در دخانیات است، تولید واسطه های مربوط به ماکروفاژهای آلوئولار از جمله سایتوکین ضدالتهابی اینترلوکین 10 (il-10) را تعدیل نماید. هدف مطالعه حاضر تاثیر یک دوره مصرف نانوکپسول سیاه دانه بر آسیب شناسی بافتی و سطوح il-10 بافت ریه رت های در معرض nnk بود.مواد و روش ها: در مطالعه تجربی حاضر 46 سر موش صحرایی بالغ نژاد ویستار به صورت تصادفی به پنج گروه مکمل، مکمل+nnk، nnk، حلال و کنترل دسته بندی شدند. گروه های تحت القای nnk به صورت زیرجلدی یک بار در هفته و به میزان 12/5میلی گرم بر کیلوگرم وزن بدن در 12 هفته nnk دریافت کردند. گروه های مکمل نیز به مدت 12 هفته نانوکپسول سیاه دانه مصرف نمودند. مقادیر سطوح il-10 در بافت هموژنیزه شده ریه با روش الایزا اندازه گیری شد. برای تجزیه و تحلیل داده ها از آنالیز واریانس یک طرفه و توکی استفاده شد.یافته ها: قرارگرفتن در معرض nnk موجب افزایش سطوح il10 در مقایسه با گروه حلال شد، گرچه از نظر آماری معنی دار نبود (p≥0/05). ضمناً یک دوره مصرف نانوکپسول سیاه دانه موجب افزایش معنی دار سطوح il-10 گروه مکمل+nnk و گروه مکمل در مقایسه با گروه nnk شد (به ترتیب p=0.038, p=0.002). علاوه بر این با بررسی اثر مکمل نانوکپسول سیاه دانه بر سطوح il-10 بافت ریه نشان داده شد که سطوح این متغیر در بافت ریه رت ها نسبت به گروه حلال افزایش معنی داری داشته است (0/001=p)نتیجه گیری: به نظر می رسد مصرف نانوکپسول سیاه دانه در مهار آثار التهابی ناشی از القای nnk با افزایش سطوح il-10، نقش موثری دارد.

Study of Protective Effect of Nigella Sativa Nanocapsules on Tissue Pathology and IL-10 levels in Lung Tissues Exposed to NNK

Aims: It is assumed that ketone derived from tobacco (NNK) which is the most important carcinogen in tobacco modulates alveolar macrophage mediator production, such as antiinflammatory cytokine IL10. The aims of this study were to investigate the effect of a Nigella sativa on tissue pathology and IL10 levels in lung tissues exposed to NNKMaterials Methods: In this experimental study, 46 Wistar rats of supplement, supplement+NNK, NNK, solvent and control. NNKinduced groups received NNK subcutaneously one day per week at a rate of 12.5 mg per kg body weight. Supplemented groups also consumed Nigella sativa Nanocapsules for 12 weeks. Levels of IL10 in homogenized lung tissue were measured by ELISA. To analyze the data; ANOVA and Tukey test were used at a significance level of p le;005.Findings: Exposure to NNK increased levels of IL10 compared with the solvent group, although not statistically significant (p ge;005). Meanwhile, a period of consumption of Nigella sativa Nanocapsules significantly increased levels of IL10 in NNK + supplement and the supplement groups compared to NNK group (p=0.038, p=0.002; respectively). In addition, the effect of Nigella sativa Nanocapsules on IL10 levels of lung tissue was shown that the levels of this variable were significantly higher than the solvent group (p=0.001).Conclusion: Generally it could be confirmed that consumption of Nigella sativa Nanocapsules plays an important role in the inhibition of inflammation induced by NNK via Increase of IL10 activity.Aims: It is assumed that ketone derived from tobacco (NNK) which is the most important carcinogen in tobacco modulates alveolar macrophage mediator production, such as antiinflammatory cytokine IL10. The aims of this study were to investigate the effect of a Nigella sativa on tissue pathology and IL10 levels in lung tissues exposed to NNKMaterials Methods: In this experimental study, 46 Wistar rats of supplement, supplement+NNK, NNK, solvent and control. NNKinduced groups received NNK subcutaneously one day per week at a rate of 12.5 mg per kg body weight. Supplemented groups also consumed Nigella sativa Nanocapsules for 12 weeks. Levels of IL10 in homogenized lung tissue were measured by ELISA. To analyze the data; ANOVA and Tukey test were used at a significance level of p le;005.Findings: Exposure to NNK increased levels of IL10 compared with the solvent group, although not statistically significant (p ge;005). Meanwhile, a period of consumption of Nigella sativa Nanocapsules significantly increased levels of IL10 in NNK + supplement and the supplement groups compared to NNK group (p=0.038, p=0.002; respectively). In addition, the effect of Nigella sativa Nanocapsules on IL10 levels of lung tissue was shown that the levels of this variable were significantly higher than the solvent group (p=0.001).Conclusion: Generally it could be confirmed that consumption of Nigella sativa Nanocapsules plays an important role in the inhibition of inflammation induced by NNK via Increase of IL10 activity.