اثر عصاره هیدروالکلی پرسیاوشان (adiantum capillus veneris) بر افسردگی و اضطراب ناشی از استرس حاد در موش‌های سوری نر

زﻣﯿﻨﻪ وﻗﻮع ﺣﻮادت اﺳﺘﺮس زای ﺷﺪﯾﺪ، ﺑﺎ ﺧﻄﺮ اﺑﺘﻠﺎ ﺑﻪ اﺧﺘﻠﺎﻟﺎت روﺣﯽ و رواﻧﯽ از ﻗﺒﯿﻞ اﻓﺴﺮدﮔﯽ و اﺿﻄﺮاب ﻫﻤﺮاه اﺳﺖ. ﺑﺎ ﺗﻮﺟﻪ ﺑﻪ ﻋﻮارض ﻣﻨﻔﯽ داروﻫﺎی ﺿﺪاﻓﺴﺮدﮔﯽ و ﺿﺪاﺿﻄﺮاب، ﺗﻮﺟﻪ ﻣﺤﻘﻘﺎن ﺑﻪ ﺗﺮﮐﯿﺒﺎت ﻃﺒﯿﻌﯽ، ﻣﻌﻄﻮف ﺷﺪه اﺳﺖ. ﻫﺪف ﻣﻄﺎﻟﻌﻪ ﺣﺎﺿﺮ، ﺑﺮرﺳﯽ ﺗﺄﺛﯿﺮ ﻋﺼﺎره ﭘﺮﺳﯿﺎوﺷﺎن ﺑﺮ اﻓﺴﺮدﮔﯽ و اﺿﻄﺮاب ﻧﺎﺷﯽ از اﺳﺘﺮس ﺣﺎد در ﻣﻮشﻫﺎی ﺳﻮری ﻧﺮ ﺑﻮد. روش ﮐﺎر در ﻣﻄﺎﻟﻌﻪ ﺗﺠﺮﺑﯽ ﺣﺎﺿﺮ 40 ﻣﻮش ﺳﻮری ﻧﺮ ﻧﮋاد balb/c ﺑﻪ ﺻﻮرت ﺗﺼﺎدﻓﯽ در ﭘﻨﺞ ﮔﺮوه ﺷﺎﻣﻞ: ﮐﻨﺘﺮل( ﺗﺤﺖ اﺳﺘﺮس ﺣﺎد درﯾﺎﻓﺖﮐﻨﻨﺪه ﻧﺮﻣﺎلﺳﺎﻟﯿﻦ)، ﻣﺪاﺧﻠﻪ (ﺗﺤﺖ اﺳﺘﺮس ﺣﺎد درﯾﺎﻓﺖﮐﻨﻨﺪه ﻋﺼﺎره ﭘﺮﺳﯿﺎوﺷﺎن در دوزﻫﺎی 200 ،100 و 400 (mg/kg)) و ﮐﻨﺘﺮل ﻣﺜﺒﺖ (ﺗﺤﺖ اﺳﺘﺮس ﺣﺎد درﯾﺎﻓﺖﮐﻨﻨﺪه دﯾﺎزﭘﺎم) ﻗﺮار ﮔﺮﻓﺘﻨﺪ. اﺳﺘﺮس ﺣﺎد، از ﻃﺮﯾﻖ ﺷﻮک اﻟﮑﺘﺮﯾﮑﯽ (0/5 ﻣﯿﻠﯽآﻣﭙﺮ، 2 دﻗﯿﻘﻪ) و ﺑﯽﺣﺮﮐﺘﯽ در دﺳﺘﮕﺎه ﻣﺤﺪودﮐﻨﻨﺪه (2 ﺳﺎﻋﺖ) ﺑﺮای ﯾﮏﺑﺎر اﯾﺠﺎد ﺷﺪ. ﻋﺼﺎره و دارو ﺑﻪ ﻣﺪت 21 روز و ﺑﻪﺻﻮرت درون ﺻﻔﺎﻗﯽ ﺗﺠﻮﯾﺰ ﺷﺪ و ﺳﭙﺲ ﻣﯿﺰان ﻓﻌﺎﻟﯿﺖ ﺣﺮﮐﺘﯽ، اﺿﻄﺮاب و اﻓﺴﺮدﮔﯽ ﺗﻮﺳﻂ آزﻣﻮنﻫﺎی ﺷﻨﺎی اﺟﺒﺎری، ﻣﺎز ﺑﻪ ﻋﻠﺎوهای ﻣﺮﺗﻔﻊ(epm) و ﺻﻔﺤﻪ ﺑﺎز ارزﯾﺎﺑﯽ ﮔﺮدﯾﺪ.ﯾﺎﻓﺘﻪﻫﺎ در ﻣﻮشﻫﺎی ﺗﺤﺖ اﺳﺘﺮس ﺣﺎد، دوزﻫﺎی ﻣﺨﺘﻠﻒ ﻋﺼﺎره ﭘﺮﺳﯿﺎوﺷﺎن، ﺳﺒﺐ ﮐﺎﻫﺶ ﻣﻌﻨﯽدار در ﻣﺪت زﻣﺎن ﺑﯽﺣﺮﮐﺘﯽ ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه ﮐﻨﺘﺮل در آزﻣﻮن ﺷﻨﺎی اﺟﺒﺎری ﺷﺪ (p<0/05). ﻫﻢﭼﻨﯿﻦ دوز mg/kg 400 ﻋﺼﺎره ﭘﺮﺳﯿﺎوﺷﺎن، ﺳﺒﺐ اﻓﺰاﯾﺶ ﻣﻌﻨﯽدار در درﺻﺪ ورود ﺑﻪ ﺑﺎزوﻫﺎی ﺑﺎز و ﮐﺎﻫﺶ ﻣﻌﻨﯽدار ﺗﻌﺪاد دﻓﻌﺎت ورود ﺑﻪ ﺑﺎزوﻫﺎی ﺑﺴﺘﻪ در epm ﻧﺴﺒﺖ ﺑﻪ ﮐﻨﺘﺮل ﮔﺮدﯾﺪ (p<0/05). دوزﻫﺎی ﻣﺨﺘﻠﻒ ﻋﺼﺎره ﭘﺮﺳﯿﺎوﺷﺎن ﺑﺮ ﺗﻌﺪاد دﻓﻌﺎت ﻋﺒﻮر از ﺧﻄﻮط ﺻﻔﺤﻪ ﺑﺎز ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه ﮐﻨﺘﺮل اﺛﺮ ﻣﻌﻨﯽدار ﻧﺪاﺷﺖ. ﻧﺘﯿﺠﻪﮔﯿﺮی ﻋﺼﺎره ﭘﺮﺳﯿﺎوﺷﺎن اﺛﺮات ﺿﺪاﻓﺴﺮدﮔﯽ و ﺿﺪاﺿﻄﺮاﺑﯽ را در ﻣﻮشﻫﺎی ﺗﺤﺖ اﺳﺘﺮس ﺣﺎد ﻧﺸﺎن ﻣﯽدﻫﺪ.

Effect of hydroalcoholic extract of Adiantum capillus veneris on depression and anxiety induced by acute stress in male mice

Introduction:Severe stressful incidents are associated with the risk of mental disorders such as depression and anxiety. Regarding the adverse effects of antidepressants/anxiety drugs, researcher’s attention have been focused on finding natural ingredients. The aim of the present study was to investigate the effect of Adiantum capillus veneris extract on depression and anxiety induced by acute stress in male rats.Materials and Methods: In this experimental study, 40 male Balb/C mice were randomly divided into 5 groups including control group (under acute stress receiving normal saline), intervention groups (under acute stress receiving extract of Adiantum capillus venerisat doses of 100, 200 and 400 mg/kg), and positive control (under acute stress receiving diazepam). Acute stress was induced by electric shock (0.5 mA, 2 min) and immobilization in restrainer (2 hours) for one time. Extract and drug were administered intraperitoneally for 21 days and then anxiety, depression and Motor activity were evaluated by forced swimming, elevated plus maze (EPM) and open filed tests respectively.Results: Treatment of stressed mice with different doses of Adiantum capillus veneris extractsignificantly decreased the immobility time in the forced swimming test in comparison with the control group (P <0.05). Treatment of stressed mice with 400 mg/kg of Adiantum capillus veneris extractsignificantly increased the entry into the open arms and significantly decreased the number of entery into the closed arms of EPM (P<0.05). Different doses of extract had no significant effect on the number of linecrossing in the open field test as compared with the control group.Conclusion: Adiantum capillus veneris extract showed antidepressant and antianxiolytic effects in mice under acute stress.