molecular and morphological evidence for two clonostachys and one sesquicillium species new to iran

To identify some fungal species associated with root–knot nematode (meloidogyne species) or inhabiting the rhizosphere of kiwifruit trees (actinidia chinensis), infected roots and rhizospheric soil samples were collected from kiwifruit orchards in guilan province (north of iran). for fungal isolation from soil, one g of each soil sample was suspended in 100 ml of sterile distilled water. after making serial dilutions, 100 µl of each dilution was spread onto pda-rose bengal agar supplemented with streptomycin. for isolation from nematodes, egg masses were surface-sterilized in 0.5% sodium hypochlorite, transferred to the same culture medium, and incubated at 25 °c in the dark for 10 days. the isolates were characterized using the morphological assessment and dna sequence analyses of the internal transcribed spacer (its), translation elongation factor 1-α (tef1), and beta-tubulin (tub2) regions. some isolates produced monomorphic or dimorphic conidiophores typical of members of the clonostachys and sesquicillium genera. morphological and molecular data confirmed that, the isolates represented three species, including clonostachys chloroleuca (from rhizospheric soli), c. rogersoniana (from a meloidogyne sp. egg sac), and sesquicillium aff. essexcoheniae (from rhizospheric soil). to the best of the authors’ knowledge, this is the first report of these species from iran.

molecular and morphological evidence for two clonostachys and one sesquicillium species new to iran

to identify some fungal species associated with root–knot nematode (meloidogyne species) or inhabiting the rhizosphere of kiwifruit trees (actinidia chinensis), infected roots and rhizospheric soil samples were collected from kiwifruit orchards in guilan province (north of iran). for fungal isolation from soil, one g of each soil sample was suspended in 100 ml of sterile distilled water. after making serial dilutions, 100 µl of each dilution was spread onto pda-rose bengal agar supplemented with streptomycin. for isolation from nematodes, egg masses were surface-sterilized in 0.5% sodium hypochlorite, transferred to the same culture medium, and incubated at 25 °c in the dark for 10 days. the isolates were characterized using the morphological assessment and dna sequence analyses of the internal transcribed spacer (its), translation elongation factor 1-α (tef1), and beta-tubulin (tub2) regions. some isolates produced monomorphic or dimorphic conidiophores typical of members of the clonostachys and sesquicillium genera. morphological and molecular data confirmed that, the isolates represented three species, including clonostachys chloroleuca (from rhizospheric soli), c. rogersoniana (from a meloidogyne sp. egg sac), and sesquicillium aff. essexcoheniae (from rhizospheric soil). to the best of the authors’ knowledge, this is the first report of these species from iran.