Evaluation of COI and ITS2 sequences ability to distinguish Lysiphlebus fabarum and Lysiphlebus confusus (Hymenoptera: Aphidiidae)

All wasp species in the genus lysiphlebus f?rster are solitary endoparasitoids of aphids and considered a taxonomically difficult group. in this study a part of coi and its2 was used to distinguish two closely related species l. fabarum (marshall) and l. confusus tremblay & eady in iran. the sequencing with a similarity about 99.5% showed coi's inability to separate the species l. fabarum and l. confusus. the its2 region of the rdna partly separated the species with the similarity of 96.5-98.5%. the result is indicative of dna barcoding deficiency in the identification of the species, as the two gene regions fail to efficiently separate the species.