Modulating Effects of Aerobic Exercise on Apoptosis Markers in Cardiac Cells of Diabetic Rats With Morphine Deprivation Syndrome

Background: diabetes and morphine play are critical in causing cardiac apoptosis. objective: this study investigates the effect of eight weeks of aerobic training on apoptosis indices in the heart tissue of diabetic rats with morphine withdrawal syndrome. methods: in this study, 32 male wistar rats were randomly assigned into four groups, including diabetic control, diabetic control+morphine withdrawal syndrome, diabetic+aerobic exercise, and diabetic+morphine withdrawal syndrome+aerobic exercise. subsequently, the induction of diabetes and morphine was done. the training groups participated in an increasing treadmill running program for 8 weeks (3 sessions per week). then, 24 h after the last training session, the heart tissue was sampled. the levels of bax and bcl-2 proteins in the tissue of the left ventricle of the rat heart were measured by the elisa method. at the significance level of p<0.05, the data was analyzed using the one-way analysis of variance and the tukey post hoc test. findings: the amount of bax was significantly higher in the groups of diabetic control+morphine withdrawal syndrome (p=0.002) and diabetic+morphine withdrawal syndrome+aerobic exercise (p=0.008) compared to the diabetic control group. also, the level of bcl-2 protein in the diabetic control group was significantly lower than in the diabetic+aerobic exercise (p=0.004) and diabetes+morphine withdrawal syndrome+aerobic exercise (p=0.04) groups. in addition, the bax/bcl-2 ratio was lower in the diabetic+aerobic exercise (p=0.001) and diabetes+morphine withdrawal syndrome+aerobic exercise (p=0.045) groups compared to the diabetic control group. conclusion: the results suggest that aerobic exercise can be an effective non-pharmacological way to reduce the effects of apoptosis on heart tissue cells in diabetic and diabetic rats with morphine withdrawal syndrome.